7CIF
Crystal structure of L-methionine decarboxylase from Streptomyces sp.590 (internal aldimine form).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.631, 147.499, 53.904 |
| Unit cell angles | 90.00, 99.87, 90.00 |
Refinement procedure
| Resolution | 19.890 - 1.800 |
| R-factor | 0.19174 |
| Rwork | 0.190 |
| R-free | 0.23064 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.555 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.054 | 0.499 |
| Rpim | 0.077 | 0.704 |
| Number of reflections | 86707 | 25929 |
| <I/σ(I)> | 8.4 | 1.6 |
| Completeness [%] | 92.7 | 91.6 |
| Redundancy | 1.8 | 1.87 |
| CC(1/2) | 0.996 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M MMT buffer pH 6.0, 23.5 % (w/v) PEG1500 |
