7CI1
Crystal structure of AcrVA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 65 |
| Unit cell lengths | 84.287, 84.287, 264.171 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.970 - 2.300 |
| R-factor | 0.2303 |
| Rwork | 0.229 |
| R-free | 0.25130 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.690 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.068 | 0.862 |
| Rmeas | 0.072 | 0.930 |
| Rpim | 0.022 | 0.342 |
| Number of reflections | 46743 | 2349 |
| <I/σ(I)> | 31.5 | 2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 10.1 | 7 |
| CC(1/2) | 1.000 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 0.085 M Hepes pH7.5, 1.7% PEG400, 1.7 M (NH4)2SO4, 15% Glycerol |
