7CHU
Geobacillus virus E2 - ORF18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.842, 150.808, 84.707 |
| Unit cell angles | 90.00, 95.14, 90.00 |
Refinement procedure
| Resolution | 36.698 - 2.008 |
| R-factor | 0.1598 |
| Rwork | 0.158 |
| R-free | 0.19580 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.700 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.140 | 0.540 |
| Number of reflections | 101736 | 2846 |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 95.8 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES, 0.2M sodium chloride, pH 7.5, 25% (w/v) polyethylene glycol 4000 |
