7CHC

Crystal structure of the SARS-CoV-2 S RBD in complex with BD-629 Fab and BD-368-2 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-06-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	101.900, 105.700, 110.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.960 - 2.710
R-factor	0.2157
Rwork	0.212
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6moj
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Number of reflections	33124	1612
<I/σ(I)>	12.49
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.992	0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Imidazole pH 7.0 and 20% w/v Polyethylene glycol 6000