7CGK
Crystal Structure of PUF-8 in Complex with PBE-RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.796, 89.796, 117.551 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.002 - 2.400 |
| R-factor | 0.1894 |
| Rwork | 0.187 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q0q |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.976 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 (V716.1) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10-2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.112 | 0.048 | 0.601 |
| Rmeas | 0.116 | 0.051 | 0.626 |
| Rpim | 0.031 | 0.014 | 0.172 |
| Total number of observations | 243899 | ||
| Number of reflections | 19494 | 1107 | 961 |
| <I/σ(I)> | 5.5 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 12.5 | 11 | 12.3 |
| CC(1/2) | 0.999 | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 1M LiCl, 0.1M Citric acid (pH 5.0) and 15% PEG6000 |
