7CG8
Structure of the sensor domain (short construct) of the anti-sigma factor RsgI4 in Pseudobacteroides cellulosolvens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.590, 71.050, 100.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.490 - 1.500 |
| R-factor | 0.1608 |
| Rwork | 0.160 |
| R-free | 0.18790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cg5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.896 |
| Data scaling software | XDS (20191211) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.960 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.074 | 0.837 |
| Number of reflections | 120016 | 8817 |
| <I/σ(I)> | 11.44 | 1.8 |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 3.48 | |
| CC(1/2) | 0.998 | 0.664 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2 M ammonium acetate, 0.1 M Bis-Tris (pH 6.5), 25% PEG3350 |
