7CFZ
SH3 domain of NADPH oxidase activator 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2018-02-04 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 30.596, 49.014, 72.868 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.680 - 1.890 |
R-factor | 0.2298 |
Rwork | 0.227 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k28 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.695 |
Data scaling software | HKL-2000 (1.13_2998) |
Phasing software | PHASER (1.13_2998) |
Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.960 |
High resolution limit [Å] | 1.885 | 1.885 |
Rmerge | 0.106 | 0.642 |
Number of reflections | 9201 | 225 |
<I/σ(I)> | 27.2 | 2.08 |
Completeness [%] | 98.6 | 98.22 |
Redundancy | 5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES pH 7.5, 0.2M Magnesium chloride hexahydrate, 25% PEG 3,350 |