7CFY

Crystal Structure of YbeA CP74 W48F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-15
Detector	RAYONIX MX300-HS
Wavelength(s)	0.99984
Spacegroup name	P 1
Unit cell lengths	39.137, 57.833, 69.629
Unit cell angles	67.17, 90.05, 90.15

Refinement procedure

Resolution	23.723 - 2.405
R-factor	0.226
Rwork	0.217
R-free	0.30670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zyo
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	23.723	2.500
High resolution limit [Å]	2.405	5.180	2.410
Rmerge	0.098	0.037	0.488
Rmeas	0.122	0.050	0.607
Rpim	0.072	0.033	0.356
Number of reflections	20734	2126	1994
<I/σ(I)>	9.1
Completeness [%]	95.6	97.9	90.1
Redundancy	2.7	2.4	2.4
CC(1/2)		0.996	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	0.1M Sodium acetate pH 4.5, 0.1M Calcium acetate hydrate, 10% PEG 4000