7CFG
Structure of the transmembrane domain of the bacterial CNNM/CorC family Mg2+ transporter in complex with Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 57.570, 83.120, 98.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.330 - 3.200 |
| R-factor | 0.2322 |
| Rwork | 0.227 |
| R-free | 0.27870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.194 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 3.390 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 3734 | 1109 |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 90.3 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.978 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4 | 291 | 10 mM ZnCl2, 100 mM sodium acetate (pH 4.0), 40% polyethylene glycol 200 |
