7CFE

Crystal structure of RsmG methyltransferase of M. tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	44.980, 63.880, 72.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.260 - 2.020
R-factor	0.1899
Rwork	0.188
R-free	0.22580
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	iMOSFLM
Data scaling software	iMOSFLM
Phasing software	AutoSol
Refinement software	PHENIX ((1.18_3845: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.260	2.070
High resolution limit [Å]	2.020	2.020
Number of reflections	13090	799
<I/σ(I)>	6.6
Completeness [%]	98.7
Redundancy	5.6
CC(1/2)	0.991	0.532

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		296	0.1 M Bis-tris pH 7.5, 0.18 M Na/K phosphate and 28% PEG 3350