7CFB

Drosophila melanogaster Krimper eTud1 apo structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	60.458, 87.692, 90.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.440 - 2.100
R-factor	0.2049
Rwork	0.204
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cfc
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.134	1.029
Rmeas	0.148
Rpim	0.062	0.472
Number of reflections	28640	2795
<I/σ(I)>	12.5	1.4
Completeness [%]	99.8	100
Redundancy	5.4	5.5
CC(1/2)	0.995	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M HEPES pH 7.5, 1.26 M Ammonium Sulfate