7CF7
Crystal Structure of YbeA CP74 W72F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-11-08 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.99984 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.628, 38.698, 52.325 |
| Unit cell angles | 75.29, 73.80, 76.47 |
Refinement procedure
| Resolution | 23.608 - 1.618 |
| R-factor | 0.1904 |
| Rwork | 0.189 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zyo |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.680 |
| High resolution limit [Å] | 1.618 | 3.490 | 1.620 |
| Rmerge | 0.055 | 0.025 | 0.385 |
| Rmeas | 0.069 | 0.035 | 0.489 |
| Rpim | 0.042 | 0.024 | 0.298 |
| Number of reflections | 32081 | 3219 | 3144 |
| <I/σ(I)> | 17.1 | ||
| Completeness [%] | 93.9 | 94.3 | 92.7 |
| Redundancy | 2.7 | 2.2 | 2.4 |
| CC(1/2) | 0.997 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Sodium cacodylate pH 6.5, 25% PEG 4000 |
