7CEL
CBH1 (E217Q) IN COMPLEX WITH CELLOHEXAOSE AND CELLOBIOSE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 296 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-09-02 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 83.700, 84.100, 111.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.500 - 1.900 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.21500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.400 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.059 | 0.155 |
| Number of reflections | 30176 | |
| <I/σ(I)> | 6.6 | 4.3 |
| Completeness [%] | 96.5 | 81.6 |
| Redundancy | 5.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | MES | 100 (mM) | |
| 3 | 1 | reservoir | mPEG5000 | 18 (%(w/v)) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | azide | 0.02 (%(w/v)) |
