7CEI

THE ENDONUCLEASE DOMAIN OF COLICIN E7 IN COMPLEX WITH ITS INHIBITOR IM7 PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-02-15
Detector	FUJI
Spacegroup name	I 2 2 2
Unit cell lengths	65.440, 76.020, 119.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.300
R-factor	0.203
Rwork	0.203
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cei
RMSD bond length	0.005
RMSD bond angle	26.050 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.043	0.191
Total number of observations	38306 *
Number of reflections	12090
<I/σ(I)>	17.7	2
Completeness [%]	88.5	76.3
Redundancy	3.2	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *		15 MG/ML PROTEIN COMPLEX, 5 MM NA CITRATE, 0.25 M NH4 ACETATE, 10% PEG4000, PH 6.0 VAPOR DIFFUSION AGAINST 22.5% PEG4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	sodium citrate	5 (mM)
3	1	drop	ammonium acetate	0.25 (M)
4	1	drop	PEG4000	10 (%)
5	1	reservoir	PEG4000	22.5 (%)