7CEE
Crystal structure of mouse neuroligin-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.842, 167.142, 177.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.664 - 2.763 |
| R-factor | 0.2192 |
| Rwork | 0.217 |
| R-free | 0.25140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bix |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.871 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.810 |
| High resolution limit [Å] | 2.760 | 7.480 | 2.760 |
| Rmerge | 0.111 | 0.054 | 0.398 |
| Rmeas | 0.117 | 0.056 | 0.444 |
| Rpim | 0.037 | 0.016 | 0.190 |
| Total number of observations | 388970 | ||
| Number of reflections | 50446 | 2772 | 2443 |
| <I/σ(I)> | 4.8 | ||
| Completeness [%] | 99.1 | 99.7 | 98.7 |
| Redundancy | 7.7 | 11.4 | 4.8 |
| CC(1/2) | 0.998 | 0.518 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 15% PEG 3350, 0.2 M ammonium chloride, 0.1 M MES-Na |
