7CE0
Crystal structure of 2019-nCoV nucleocapsid C-terminal domain (CTD) protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.726, 50.310, 69.134 |
| Unit cell angles | 73.88, 89.94, 82.55 |
Refinement procedure
| Resolution | 26.820 - 1.500 |
| R-factor | 0.1773 |
| Rwork | 0.177 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cjr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.651 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.410 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Number of reflections | 103766 | 3864 |
| <I/σ(I)> | 30.265 | |
| Completeness [%] | 91.7 | |
| Redundancy | 3.4 | 3 |
| CC(1/2) | 0.996 | 0.808 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 4M Potassium formate, 0.1M BIS-TRIS propane, pH 9.0, 2% w/v Polyethylene glycol monomethyl ether 2000 |
