7CDW

Crystal Structure of Mycobacterium Tuberculosis Elongation Factor G1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97931
Spacegroup name	C 2 2 21
Unit cell lengths	101.543, 301.516, 145.865
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.950 - 3.000
R-factor	0.2078
Rwork	0.206
R-free	0.24140
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.850	3.190
High resolution limit [Å]	3.000	3.000
Rmerge	0.091
Number of reflections	83429	13613
<I/σ(I)>	11.73	2.37
Completeness [%]	96.7	97.6
Redundancy	2.92	2.88
CC(1/2)	0.996	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	20mM magnesium chloride,50mM MOPS pH 7.0,55%TacsimateTM pH 7.0 and 5mM Hexammine cobalt(III) chloride