7CDJ

Crystal structure of SARS-CoV-2 antibody P2C-1A3 with RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9796
Spacegroup name	P 61 2 2
Unit cell lengths	89.411, 89.411, 437.923
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.312 - 3.396
R-factor	0.2334
Rwork	0.231
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.312	3.480
High resolution limit [Å]	3.396	3.400
Rmerge	0.118	0.933
Number of reflections	15108	1322
<I/σ(I)>	8.8
Completeness [%]	99.7
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M lithium sulfate monohydrate, 0.1M citric acid, pH 3.5, 18% PEG 6000