7CD7

GFP-40/GFPuv complex, Form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-07-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	54.712, 103.168, 67.861
Unit cell angles	90.00, 106.28, 90.00

Refinement procedure

Resolution	47.982 - 1.704
R-factor	0.1846
Rwork	0.182
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9c 2o9u
RMSD bond length	0.014
RMSD bond angle	1.298
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	78215	3848
<I/σ(I)>	24.5	2.1
Completeness [%]	99.5
Redundancy	3.2
CC(1/2)	0.990	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	15% (w/v) PEG 4000, 200 mM MgCl2, 0.1 M Tris-HCl, pH 8.5