7CCL
Crystal structure of human BFK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 32 |
| Unit cell lengths | 116.500, 116.500, 27.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.125 - 2.700 |
| Rwork | 0.158 |
| R-free | 0.19010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ccm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.167 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.077 | 0.809 |
| Number of reflections | 11459 | 878 |
| <I/σ(I)> | 13.5 | 1.96 |
| Completeness [%] | 99.3 | 98.1 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.998 | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M Bis-Tris pH 5.5, 28% PEG 3350 |
