7CBW
Blasnase-T13A with D-asn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.940, 91.940, 231.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.029 - 1.978 |
| Rwork | 0.167 |
| R-free | 0.18740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c91 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.699 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.029 | 2.020 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.021 | 0.235 |
| Number of reflections | 93591 | 3638 |
| <I/σ(I)> | 20.5 | 2.3 |
| Completeness [%] | 98.9 | 82.6 |
| Redundancy | 23.6 | 9.4 |
| CC(1/2) | 0.999 | 0.826 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | formate |
