7CBU
Blasnase-T13A with L-Asp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.651, 92.651, 234.096 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.925 - 2.250 |
Rwork | 0.196 |
R-free | 0.21690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7c91 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.702 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.925 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Rpim | 0.041 | 0.408 |
Number of reflections | 49335 | 4447 |
<I/σ(I)> | 12.3 | 1.9 |
Completeness [%] | 99.9 | |
Redundancy | 24.6 | |
CC(1/2) | 0.999 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | formate |