7CBD
Catalytic domain of Cellulomonas fimi Cel6B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.667, 49.390, 71.004 |
| Unit cell angles | 90.00, 96.36, 90.00 |
Refinement procedure
| Resolution | 28.380 - 1.300 |
| R-factor | 0.1263 |
| Rwork | 0.126 |
| R-free | 0.15590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4avo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.863 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.038 | 0.149 |
| Number of reflections | 81834 | 3965 |
| <I/σ(I)> | 39.2 | 10.9 |
| Completeness [%] | 89.5 | 87.2 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 5 | 293 | 21% PET3350, 10 mM sodium chloride, 100 mM sodium acetate |
