7CAY
Crystal Structure of Lon N-terminal domain protein from Xanthomonas campestris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-07 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 124.925, 124.925, 55.586 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.327 - 2.800 |
| R-factor | 0.2634 |
| Rwork | 0.262 |
| R-free | 0.28760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zbo |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.569 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.170 | 44.170 | 2.950 |
| High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
| Rmerge | 0.076 | 0.026 | 0.905 |
| Rmeas | 0.081 | 0.028 | 0.963 |
| Rpim | 0.028 | 0.010 | 0.325 |
| Total number of observations | 46030 | 1426 | 6711 |
| Number of reflections | 5595 | 201 | 795 |
| <I/σ(I)> | 19.9 | 57.1 | 2.3 |
| Completeness [%] | 99.0 | 95.6 | 99.6 |
| Redundancy | 8.2 | 7.1 | 8.4 |
| CC(1/2) | 0.999 | 0.999 | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 277 | Isopropanol 35%, 200mM ammonium acetate, 100 mM Tris-Cl, pH 8.5 |
