7CAS
Versatile cis-prenyltransferase MM_0014 from Methanosarcina mazei (crystal type: free+PPi)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-12 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.779, 100.219, 130.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.040 - 2.280 |
| R-factor | 0.22192 |
| Rwork | 0.220 |
| R-free | 0.25812 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7caq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.010 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.400 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.095 | 0.697 |
| Number of reflections | 24258 | 3470 |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.9 | |
| CC(1/2) | 0.999 | 0.858 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 4 mM cobalt (II) chloride, 4 mM nickel (II) chloride, 4 mM cadmium chloride, 4 mM magnesium chloride, 0.075 M HEPES, pH7.5, 9% PEG 3350, 25% glycerol |
