7CAO

Crystal structure of red chromoprotein from Olindias formosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	I 41 2 2
Unit cell lengths	116.476, 116.476, 161.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.570 - 2.050
R-factor	0.1931
Rwork	0.192
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gb3
RMSD bond length	0.016
RMSD bond angle	2.096
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.570	49.570	2.170
High resolution limit [Å]	2.050	6.110	2.050
Rmerge	0.039	0.020	0.540
Rmeas	0.041	0.021	0.571
Total number of observations	315105
Number of reflections	34497	1445	5324
<I/σ(I)>	31.29	83.86	3.65
Completeness [%]	98.3	98.8	95.2
Redundancy	9.134	7.919	9.19
CC(1/2)	1.000	1.000	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG3350, sodium chloride, citrate, pH5.4