7C9J
Transglutaminase from Geobacillus stearothermophilus (without C-terminal extension)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-07 |
Detector | DECTRIS EIGER2 X 4M |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 94.090, 94.090, 158.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.450 - 2.100 |
R-factor | 0.1876 |
Rwork | 0.186 |
R-free | 0.21360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p8i |
RMSD bond length | 0.009 |
RMSD bond angle | 0.983 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.220 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.043 | 0.517 |
Rmeas | 0.047 | 0.618 |
Number of reflections | 15940 | 4544 |
<I/σ(I)> | 21.16 | 1.89 |
Completeness [%] | 98.6 | 91.8 |
Redundancy | 5.8 | 2.98 |
CC(1/2) | 0.999 | 0.743 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 20000, 2-(N-morpholino)ethanesulfonic acid |