7C7Z

Crystal structure of the flagellar junction protein FlgL from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-05
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	108.133, 108.133, 184.146
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.060
R-factor	0.2291
Rwork	0.228
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yti
RMSD bond length	0.010
RMSD bond angle	1.117
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.100
High resolution limit [Å]	3.050	3.050
Rmerge	0.094	0.553
Number of reflections	21299	1044
<I/σ(I)>	28.9	4.7
Completeness [%]	99.6	99.9
Redundancy	5.9	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	PEG 6000, citric acid