7C7Y
C-terminal domain of B. cereus TubY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.053, 40.282, 80.669 |
| Unit cell angles | 101.97, 94.22, 98.52 |
Refinement procedure
| Resolution | 26.160 - 2.600 |
| R-factor | 0.22674 |
| Rwork | 0.224 |
| R-free | 0.27720 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.519 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.064 | 0.330 |
| Number of reflections | 9894 | 485 |
| <I/σ(I)> | 27.2 | |
| Completeness [%] | 98.0 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG400 |
