7C7U

Biofilm associated protein - BSP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97892
Spacegroup name	P 1 21 1
Unit cell lengths	51.737, 167.726, 89.316
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.440 - 1.930
R-factor	0.21
Rwork	0.208
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c7r
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.440	2.000
High resolution limit [Å]	1.930	1.930
Number of reflections	111309	9729
<I/σ(I)>	16.71
Completeness [%]	97.8
Redundancy	4.7
CC(1/2)	0.999	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.1M Sodium Citrate pH5.0, 10% 2-propanol, 26% PEG400