7C7R

Biofilm associated protein - B domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978920
Spacegroup name	P 41 21 2
Unit cell lengths	171.171, 171.171, 102.503
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.100 - 3.070
R-factor	0.1935
Rwork	0.192
R-free	0.22430
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	3.180
High resolution limit [Å]	3.070	3.070
Number of reflections	27970	2317
<I/σ(I)>	25.33
Completeness [%]	96.4
Redundancy	2
CC(1/2)	0.999	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.1M Sodium Cacodylate pH 6.5, 40% MPD, 5% PEG8000