7C5X
Crystal structure of the iota-carbonic anhydrase from eukaryotic microalga complexed with bicarbonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2019-12-02 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.9000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.941, 194.298, 58.342 |
| Unit cell angles | 90.00, 96.88, 90.00 |
Refinement procedure
| Resolution | 48.570 - 2.550 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c5y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.877 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.580 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.185 | 0.393 |
| Number of reflections | 33639 | |
| <I/σ(I)> | 8.36 | 3.83 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 20%(w/v) PEG4000, 20%(v/v) i-propanol, 0.1 M sodium citrate pH 5.6 |
