7C5K
Crystal Structure of C150S mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli complexed with G3P at 2.69 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.378, 90.378, 342.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.223 - 2.680 |
| R-factor | 0.16171 |
| Rwork | 0.158 |
| R-free | 0.23677 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c5f |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.604 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.730 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.135 | 0.487 |
| Number of reflections | 41105 | 41105 |
| <I/σ(I)> | 15.17 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 100mM sodium PBS pH 6.1, 16%(w/v) PEG 1000, 200mM MgCl2 |
