7C50
Crystal structure of a Simpl-like protein from Campylobacter jejuni (selenomethionine-incorporated protein)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-04-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 82.675, 82.675, 202.043 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.210 - 1.930 |
| R-factor | 0.2201 |
| Rwork | 0.218 |
| R-free | 0.24960 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.153 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 20244 | 1001 |
| <I/σ(I)> | 41.1 | 4.1 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 5.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | polyethylene glycol 8000, calcium acetate, imidazole |
