7C4X

Crystal structure of germination protease from the spore-forming bacterium Paenisporosarcina sp. TG-20 in its inactive form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9796
Spacegroup name	P 32 2 1
Unit cell lengths	156.617, 156.617, 95.497
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.210 - 2.500
R-factor	0.2027
Rwork	0.201
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c8b
RMSD bond length	0.009
RMSD bond angle	0.994
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	46339	2020
<I/σ(I)>	29.4
Completeness [%]	98.9
Redundancy	13.2
CC(1/2)	0.779	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	3.5 M sodium formate and 0.1 M Bis-Tris propane pH 7.0 with 2.8 M sodium acetate