7C4K
Ancestral L-amino acid oxidase (AncLAAO-N5) ligand free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-10 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 131.897, 131.897, 191.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.800 - 2.400 |
| R-factor | 0.1734 |
| Rwork | 0.171 |
| R-free | 0.23050 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.923 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.810 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.125 | 0.674 |
| Number of reflections | 33315 | 4796 |
| <I/σ(I)> | 25.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 26.4 | |
| CC(1/2) | 0.999 | 0.967 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 295 | 1.0M NaCl, 0.1M citric acid pH 3.5 |
