7C3A

Ferredoxin reductase in carbazole 1,9a-dioxygenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-29
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	158.692, 158.692, 81.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.670 - 2.600
R-factor	0.2509
Rwork	0.249
R-free	0.29150
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.284
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	SOLVE
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.100	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.088	0.408
Number of reflections	32288	3208
<I/σ(I)>	8.7	3.8
Completeness [%]	99.1	99.9
Redundancy	5.9	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.16 mM calcium acetate or magnesium acetate, 8-12%(w/v) PEG 8000, 0.2 M sodium iodide, 0.08 M MES
1	VAPOR DIFFUSION, HANGING DROP		277	0.16 mM calcium acetate or magnesium acetate, 8-12%(w/v) PEG 8000, 0.2 M sodium iodide, 0.08 M MES
1	VAPOR DIFFUSION, HANGING DROP		277	0.16 mM calcium acetate or magnesium acetate, 8-12%(w/v) PEG 8000, 0.2 M sodium iodide, 0.08 M MES