7C2P
Structure of Egk Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-23 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.000034 |
Spacegroup name | P 1 |
Unit cell lengths | 24.840, 37.920, 54.770 |
Unit cell angles | 83.76, 78.35, 70.22 |
Refinement procedure
Resolution | 35.650 - 2.100 |
R-factor | 0.2311 |
Rwork | 0.230 |
R-free | 0.26270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uj0 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.890 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.000 | 2.220 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.160 | |
Number of reflections | 9535 | 1392 |
<I/σ(I)> | 3.11 | |
Completeness [%] | 88.3 | |
Redundancy | 1.7 | |
CC(1/2) | 0.902 | 0.670 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium tartrate dibasic dehydrate, 20% w/v PEG 3350 |