7C2H

Crystal Structure of the Thorarchaeota 2DGel3/rabbit actin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-29
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	49.915, 98.644, 62.448
Unit cell angles	90.00, 94.02, 90.00

Refinement procedure

Resolution	31.206 - 2.352
R-factor	0.2037
Rwork	0.202
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c2g
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.440
High resolution limit [Å]	2.352	5.080	2.360
Rmerge	0.135	0.052	0.584
Rmeas	0.146	0.057	0.643
Rpim	0.055	0.021	0.263
Number of reflections	23279	2524	1976
<I/σ(I)>	8.1
Completeness [%]	93.7	99.3	81.4
Redundancy	6.8	7.2	5.5
CC(1/2)		0.998	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M CHES pH 9.5 20% w/v polyethylene glycol 8000