7C28

Unusual quaternary structure of a homodimeric synergistic toxin from mamba snake venom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97640
Spacegroup name	I 2 2 2
Unit cell lengths	52.439, 55.982, 102.818
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.271 - 2.400
R-factor	0.2459
Rwork	0.241
R-free	0.28630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ff4
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.600
High resolution limit [Å]	2.400	2.400
Number of reflections	6110	1517
<I/σ(I)>	7.2
Completeness [%]	99.0
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	0.2 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dihydrate pH 5.6, 2.0 M ammonium sulfate