7C26
Glycosidase Wild Type at pH4.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-06 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 105.345, 105.345, 90.328 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.156 - 1.803 |
Rwork | 0.176 |
R-free | 0.20610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z3a |
RMSD bond length | 0.011 |
RMSD bond angle | 1.678 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.160 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmeas | 0.082 | 1.617 |
Number of reflections | 41276 | 6071 |
<I/σ(I)> | 0.286 | 0.023 |
Completeness [%] | 87.1 | 80.6 |
Redundancy | 25.8 | 26.4 |
CC(1/2) | 0.999 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M Ammonium citrate (pH 7.0), 8% PEG monomethyl ether 2000 |