7C21
Crystal structure of Duvenhage virus phosphoprotein C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.190, 49.520, 25.280 |
| Unit cell angles | 90.00, 99.75, 90.00 |
Refinement procedure
| Resolution | 43.800 - 1.950 |
| R-factor | 0.2027 |
| Rwork | 0.198 |
| R-free | 0.26450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vyi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.830 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.800 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.216 | 1.385 |
| Number of reflections | 8380 | 1361 |
| <I/σ(I)> | 7.53 | 1.56 |
| Completeness [%] | 98.0 | 97.4 |
| Redundancy | 8.6 | 8.6 |
| CC(1/2) | 0.990 | 0.607 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH7.5, 20% PEG 8000 |
