7C1Z

ATP bound structure of Pseudouridine kinase (PUKI) from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	80.613, 47.917, 91.928
Unit cell angles	90.00, 108.95, 90.00

Refinement procedure

Resolution	30.715 - 2.096
R-factor	0.188296511997
Rwork	0.186
R-free	0.23719
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.254
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.090	2.100
Number of reflections	39242	1116
<I/σ(I)>	15.3
Completeness [%]	99.5
Redundancy	3.5
CC(1/2)	0.988	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	0.1M tri sodium citrate, 22% PEG3350