7C1L

Crystal structure of the starter condensation domain of rhizomide synthetase RzmA mutant R148A in complex with C8-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	52.907, 72.331, 108.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.454 - 1.850
R-factor	0.193
Rwork	0.191
R-free	0.22830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c1h
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.14)
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.190	60.190	1.950
High resolution limit [Å]	1.847	5.840	1.850
Rmerge	0.068	0.045	0.532
Rmeas	0.071	0.047	0.563
Rpim	0.020	0.014	0.182
Total number of observations		14831	47174
Number of reflections	36483	1301	5195
<I/σ(I)>	17.8	41.5	2.8
Completeness [%]	99.8	99.9	98.7
Redundancy	12.1	11.4	9.1
CC(1/2)	0.999	0.999	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	289	BIS-TRIS propane, PEG 6000