7C0I

Crystal structure of chimeric mutant of E3L in complex with Z-DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 32 1 2
Unit cell lengths	72.759, 72.759, 123.876
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.905 - 2.400
R-factor	0.2028
Rwork	0.202
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qbj
Data reduction software	HKL-2000 (720)
Data scaling software	HKL-2000 (720)
Phasing software	PHASER
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.049	0.037	0.469
Rmeas	0.055	0.043	0.531
Rpim	0.026	0.021	0.247
Total number of observations	66340
Number of reflections	14894	796	729
<I/σ(I)>	9.9
Completeness [%]	99.4	98.6	98.1
Redundancy	4.5	4.1	4.4
CC(1/2)		0.997	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	295	Sodium citrate pH 4.0, Ammonium sulfate, ethylene glycol