7BZL

GH127 beta-L-arabinofuranosidase HypBA1 covalently complexed with beta-L-arabinofuranose-configured cyclophellitol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.000
Spacegroup name	P 32 2 1
Unit cell lengths	77.401, 77.401, 252.864
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.030 - 2.300
R-factor	0.2184
Rwork	0.216
R-free	0.27330
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3wkx
RMSD bond length	0.007
RMSD bond angle	1.537
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.030	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.148	2.607
Rpim	0.069	0.855
Number of reflections	40192	3892
<I/σ(I)>	9.4	0.9
Completeness [%]	100.0
Redundancy	9.8
CC(1/2)	0.998	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES-NaOH (pH 6.5), 0.7 M sodium citrate