7BZG
Structure of Bacillus subtilis HxlR, wild type in complex with formaldehyde and DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.086, 109.309, 160.423 |
| Unit cell angles | 90.00, 99.76, 90.00 |
Refinement procedure
| Resolution | 33.409 - 2.900 |
| R-factor | 0.1812 |
| Rwork | 0.179 |
| R-free | 0.22310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hqe |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.699 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.095 | 0.966 |
| Number of reflections | 42387 | 2144 |
| <I/σ(I)> | 17.6 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 MES pH = 6.4, 50 mM MgCl2, 25% v/v PEG MME 550 |
