7BZ6
Mycobacterium bovis AhpC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 137.554, 137.554, 96.842 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.039 - 3.302 |
| R-factor | 0.311 |
| Rwork | 0.309 |
| R-free | 0.34400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bmx |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.083 | 0.589 |
| Rmeas | 0.085 | 0.630 |
| Rpim | 0.018 | 0.211 |
| Number of reflections | 8387 | 727 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 98.0 | 88.1 |
| Redundancy | 11.8 | 6.9 |
| CC(1/2) | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 0.1 M sodium citrate, 16% ammonium sulfate |
