7BZ5

Structure of COVID-19 virus spike receptor-binding domain complexed with a neutralizing antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97981
Spacegroup name	C 1 2 1
Unit cell lengths	195.226, 86.417, 56.827
Unit cell angles	90.00, 100.19, 90.00

Refinement procedure

Resolution	28.490 - 1.840
R-factor	0.1677
Rwork	0.167
R-free	0.19140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lzg 4tsa
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.840	1.840
Rpim	0.044	0.526
Number of reflections	80095	7992
<I/σ(I)>	16.9	1.4
Completeness [%]	99.4
Redundancy	6.7
CC(1/2)	0.993	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	0.15 M ammonium sulfate, 0.1 M MES pH 6, 15% w/v PEG 400