7BYW

Crystal structure of Acidovorax avenae L-fucose mutarotase (L-fucose-bound form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 31
Unit cell lengths	68.398, 68.398, 69.102
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.988 - 1.750
R-factor	0.2273
Rwork	0.226
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7byu
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Rmeas	0.065	1.285
Rpim	0.020	0.411
Number of reflections	36466	1945
<I/σ(I)>	17.4	1.9
Completeness [%]	99.9	97.8
Redundancy	10.3	9.6
CC(1/2)	0.999	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8 M potassium sodium tartrate, 0.1 M Tris-HCl pH 8.5, 0.5% PEGMME 5000